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A simple and rapid preparation of pure cellulose confirmed by monosaccharide compositions, delta C-13, yields and C%
Li, Q (Li, Qiang)[ 1,2 ]; Liu, Y (Liu, Yu)[ 1,3 ]
2013
Source PublicationDENDROCHRONOLOGIA
Volume31Issue:4(SI)Pages:273-278
Subtype期刊论文
Abstract

A two-step method to extract pure cellulose from tree rings is presented in this study. The method employs the reagents diglyme (diethylene glycol dimethyl ether), hydrochloric acid and sodium chlorite in a 6-h reaction using ordinary equipment. Through separation of the chemical compositions (monosaccharides) using liquid chromatography, the product of this method is shown to contain only glucose from pure cellulose. In contrast, the chemical composition of the "alpha-cellulose" extract obtained by the traditional Jayme-Wise method is primarily glucose and mannose, which may come from hemicelluloses. These results are also verified by comparing the stable carbon isotope ratio (delta C-13), yields and percentage of carbon (C%) of the product extracted by this method and "alpha-cellulose" for different tree species. The results suggest that this method is simple, rapid and appropriate for processing large numbers of small wood samples to develop tree-ring isotope series for high-resolution paleoclimatic research with softwood trees.

KeywordPure Cellulose Two-step Method Liquid Chromatography 13c Yield C%
DOI10.1016/j.dendro.2012.07.005
Indexed By其他
Language英语
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Document Type期刊论文
Identifierhttp://ir.ieecas.cn/handle/361006/9940
Collection现代环境研究室
Corresponding AuthorLiu, Y (Liu, Yu)[ 1,3 ]
Affiliation1.State Key Laboratory of Loess and Quaternary Geology, Institute of Earth Environment, Chinese Academy of Sciences, Xi’an 710075, PR China;
2.Graduate School of Environmental Studies, Nagoya University, Nagoya 464-8601, Japan;
3.School of Human Settlements and Civil Engineering, Xi’an Jiaotong University, Xi’an 710049, PR China
Recommended Citation
GB/T 7714
Li, Q ,Liu, Y . A simple and rapid preparation of pure cellulose confirmed by monosaccharide compositions, delta C-13, yields and C%[J]. DENDROCHRONOLOGIA,2013,31(4(SI)):273-278.
APA Li, Q ,&Liu, Y .(2013).A simple and rapid preparation of pure cellulose confirmed by monosaccharide compositions, delta C-13, yields and C%.DENDROCHRONOLOGIA,31(4(SI)),273-278.
MLA Li, Q ,et al."A simple and rapid preparation of pure cellulose confirmed by monosaccharide compositions, delta C-13, yields and C%".DENDROCHRONOLOGIA 31.4(SI)(2013):273-278.
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