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A simple and rapid preparation of pure cellulose confirmed by monosaccharide compositions, delta C-13, yields and C%
Li, Q (Li, Qiang)[ 1,2 ]; Liu, Y (Liu, Yu)[ 1,3 ]
2013
发表期刊DENDROCHRONOLOGIA
卷号31期号:4(SI)页码:273-278
文章类型期刊论文
摘要

A two-step method to extract pure cellulose from tree rings is presented in this study. The method employs the reagents diglyme (diethylene glycol dimethyl ether), hydrochloric acid and sodium chlorite in a 6-h reaction using ordinary equipment. Through separation of the chemical compositions (monosaccharides) using liquid chromatography, the product of this method is shown to contain only glucose from pure cellulose. In contrast, the chemical composition of the "alpha-cellulose" extract obtained by the traditional Jayme-Wise method is primarily glucose and mannose, which may come from hemicelluloses. These results are also verified by comparing the stable carbon isotope ratio (delta C-13), yields and percentage of carbon (C%) of the product extracted by this method and "alpha-cellulose" for different tree species. The results suggest that this method is simple, rapid and appropriate for processing large numbers of small wood samples to develop tree-ring isotope series for high-resolution paleoclimatic research with softwood trees.

关键词Pure Cellulose Two-step Method Liquid Chromatography 13c Yield C%
DOI10.1016/j.dendro.2012.07.005
收录类别其他
语种英语
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ieecas.cn/handle/361006/9940
专题现代环境研究室
通讯作者Liu, Y (Liu, Yu)[ 1,3 ]
作者单位1.State Key Laboratory of Loess and Quaternary Geology, Institute of Earth Environment, Chinese Academy of Sciences, Xi’an 710075, PR China;
2.Graduate School of Environmental Studies, Nagoya University, Nagoya 464-8601, Japan;
3.School of Human Settlements and Civil Engineering, Xi’an Jiaotong University, Xi’an 710049, PR China
推荐引用方式
GB/T 7714
Li, Q ,Liu, Y . A simple and rapid preparation of pure cellulose confirmed by monosaccharide compositions, delta C-13, yields and C%[J]. DENDROCHRONOLOGIA,2013,31(4(SI)):273-278.
APA Li, Q ,&Liu, Y .(2013).A simple and rapid preparation of pure cellulose confirmed by monosaccharide compositions, delta C-13, yields and C%.DENDROCHRONOLOGIA,31(4(SI)),273-278.
MLA Li, Q ,et al."A simple and rapid preparation of pure cellulose confirmed by monosaccharide compositions, delta C-13, yields and C%".DENDROCHRONOLOGIA 31.4(SI)(2013):273-278.
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