IEECAS OpenIR  > 黄土与第四纪地质国家重点实验室(2010~)
Novel Position-Specific O-18/O-16 Measurement of Carbohydrates. I. O-3 of Glucose and Confirmation of O-18/O-16 Heterogeneity at Natural Abundance Levels in Glucose from Starch in a C-4 Plant
Ma, Ran1,2; Zhu, Zhenyu1,2; Wang, Bo1,2; Zhao, Yu1,2; Yin, Xijie4; Lu, Fengyan3; Wang, Ying1,2; Su, Jing4; Hocart, Charles H.5; Zhou, Youping1,2
2018-09-04
Source PublicationANALYTICAL CHEMISTRY
Volume90Issue:17Pages:10293-10301
SubtypeArticle
AbstractThe O-18/O-16 ratio at both molecular and positional levels in the carbohydrates of higher plants is a reliable proxy for the plant growth environment, and a potential indicator of the plant photosynthetic carbon assimilation mode, and its physiological, biochemical and metabolic status. The lack of exploitable nuclear resonance in O-18 and O-16 and the extremely low O-17 abundance make the NMR-based PSIA (position-specific isotopic analysis) a significant challenge. In this Article, an alternative three-step wet chemistry based method for accessing the O-18/O-16 of glucose O-3 is presented. The O atoms (OH groups) at positions 1, 2, 5, and 6 were first protected by acetonation (converting glucose to 1,2;5,6-di-O-isopropylidene-glucofuranose). The protected glucose was then esterified at O-3 by thionoformylation. Subsequent Barton-McCombie deoxygenation quantitatively removed the O-3 from the protected sugar. Mass balance was then applied to calculate the O-18/O-16 of O-3 using the isotopic values of the protected sugar before and after the deoxygenation step. The method is innovative in that (i) isolation and purification of individual compounds for O-18 by EA/Pyrolysis/IRMS analysis is unnecessary as the reaction mixture can be analyzed on a GC/Pyrolysis/IRMS; (ii) sample quantity is dramatically reduced; and (iii) the approach to access the O-3 isotopic signal can be easily expanded to other positions within glucose and other sugars. It was shown that O-3 is enriched by 12 mUr relative to the molecular average (O-2-O-6) for a glucose of C-4 photosynthetic origin. We highlighted the potential applications of the intramolecular O isotopic heterogeneity of glucose this method revealed.
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1021/acs.analchem.8b02022
WOS KeywordCONTAINING CHEMICAL LINKAGES ; CARBON-ISOTOPE COMPOSITION ; CELLULOSE SYNTHESIS ; MASS-SPECTROMETRY ; ORGANIC-MATTER ; LEAF WATER ; OXYGEN ; DELTA-O-18 ; SUGARS ; O-18
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000444060600031
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ieecas.cn/handle/361006/5351
Collection黄土与第四纪地质国家重点实验室(2010~)
Affiliation1.Shaanxi Univ Sci & Technol, Sch Chem & Chem Engn, Isotop Chem Biol, Xian 710021, Shaanxi, Peoples R China
2.Shaanxi Univ Sci & Technol, Sch Chem & Chem Engn, Shaanxi Key Lab Chem Addit Ind, Xian 710021, Shaanxi, Peoples R China
3.Chinese Acad Sci, Inst Earth Environm, Xian 710061, Shaanxi, Peoples R China
4.SOA Third Inst Oceanog, Xiamen 361005, Peoples R China
5.Australian Natl Univ, Res Sch Biol, Acton, ACT 2601, Australia
Recommended Citation
GB/T 7714
Ma, Ran,Zhu, Zhenyu,Wang, Bo,et al. Novel Position-Specific O-18/O-16 Measurement of Carbohydrates. I. O-3 of Glucose and Confirmation of O-18/O-16 Heterogeneity at Natural Abundance Levels in Glucose from Starch in a C-4 Plant[J]. ANALYTICAL CHEMISTRY,2018,90(17):10293-10301.
APA Ma, Ran.,Zhu, Zhenyu.,Wang, Bo.,Zhao, Yu.,Yin, Xijie.,...&Zhou, Youping.(2018).Novel Position-Specific O-18/O-16 Measurement of Carbohydrates. I. O-3 of Glucose and Confirmation of O-18/O-16 Heterogeneity at Natural Abundance Levels in Glucose from Starch in a C-4 Plant.ANALYTICAL CHEMISTRY,90(17),10293-10301.
MLA Ma, Ran,et al."Novel Position-Specific O-18/O-16 Measurement of Carbohydrates. I. O-3 of Glucose and Confirmation of O-18/O-16 Heterogeneity at Natural Abundance Levels in Glucose from Starch in a C-4 Plant".ANALYTICAL CHEMISTRY 90.17(2018):10293-10301.
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