IEECAS OpenIR  > 粉尘与环境研究室
Loss of E-cadherin due to road dust PM2.5 activates the EGFR in human pharyngeal epithelial cells
Nguyen Thanh Tung1,2; Ho, Kin-Fai3; Niu, Xinyi4; Sun, Jian5; Shen, Zhenxing5; Wu, Feng6,7; Cao, Junji6,7; Hoang Ba Dung2; Tran Phan Chung Thuy8; Hsiao, Ta-Chih9; Liu, Wen-Te10,11; Chuang, Hsiao-Chi10,11,12
Corresponding AuthorLiu, Wen-Te(lion5835@gmail.com) ; Chuang, Hsiao-Chi(chuanghc@tmu.edu.tw)
2021-05-26
Source PublicationENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
ISSN0944-1344
Pages16
AbstractExposure to road dust particulate matter (PM) causes adverse health impacts on the human airway. However, the effects of road dust on the upper airway epithelium in humans remain unclear. We investigated the involvement of the epidermal growth factor receptor (EGFR) after PM with an aerodynamic diameter of < 2.5 mu m (PM2.5)-induced E-cadherin disruption of human pharyngeal epithelial cells. First, we collected road dust PM2.5 from 10 Chinese cities, including Wuhan, Nanjing, Shanghai, Guangzhou, Chengdu, Beijing, Lanzhou, Tianjin, Harbin, and Xi'an. Human pharyngeal FaDu cells were exposed to road dust PM2.5 at 50 mu g/mL for 24 h, cytotoxicity (cell viability and lactate dehydrogenase (LDH)) was assessed, and expressions of the proinflammatory interleukin (IL)-6 and high-mobility group box 1 (HMGB1) protein, receptor for advanced glycation end products (RAGE), occludin, E-cadherin, EGFR, and phosphorylated (p)-EGFR were determined. The E-cadherin gene was then knocked down to investigate EGFR activation in FaDu cells. Exposure to road dust PM2.5 resulted in a decrease in cell viability and increases in LDH and IL-6. Our data suggested that PM2.5 could decrease expressions of occludin and E-cadherin and increase expressions of EGFR and p-EGFR, which was confirmed by E-cadherin-knockdown. Our results showed a negative association between the alterations in E-cadherin and total elemental components in correlation analysis, especially S, Cl, K, Ti, Mn, Fe, Cu, Zn, and Pb. Exposure to metals in PM2.5 from road dust may lead to loss of the barrier function of the upper airway epithelium and activation of the EGFR. Our study showed the adverse effects of road dust PM2.5 on pharyngeal epithelial cells of the human upper airway.
KeywordAir pollution Allergy Chemical Metal Particulate matter Upper airway
DOI10.1007/s11356-021-14469-2
WOS KeywordFINE PARTICULATE MATTER ; GROWTH-FACTOR RECEPTOR ; SOURCE PROFILES ; OXIDATIVE STRESS ; AIR-POLLUTION ; FUGITIVE DUST ; SOURCE APPORTIONMENT ; ALLERGIC RHINITIS ; MESENCHYMAL TRANSITION ; ELEMENTAL CARBON
Indexed BySCI ; SCI
Language英语
Funding ProjectMinistry of Science and Technology of Taiwan[109-2314-B-038-093-MY3]
WOS Research AreaEnvironmental Sciences & Ecology
Funding OrganizationMinistry of Science and Technology of Taiwan
WOS SubjectEnvironmental Sciences
WOS IDWOS:000654150700004
PublisherSPRINGER HEIDELBERG
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ieecas.cn/handle/361006/16706
Collection粉尘与环境研究室
Corresponding AuthorLiu, Wen-Te; Chuang, Hsiao-Chi
Affiliation1.Taipei Med Univ, Coll Med, Int PhD Program Med, Taipei, Taiwan
2.Cho Ray Hosp, Otorhinolaryngol Dept, Ho Chi Minh City, Vietnam
3.Chinese Univ Hong Kong, JC Sch Publ Hlth & Primary Care, Hong Kong, Peoples R China
4.Xi An Jiao Tong Univ, Sch Human Settlements & Civil Engn, Xian, Peoples R China
5.Xi An Jiao Tong Univ, Dept Environm Sci & Engn, Xian, Peoples R China
6.Chinese Acad Sci, Inst Earth Environm, Key Lab Aerosol Chem & Phys, Xian, Peoples R China
7.Chinese Acad Sci, Inst Earth Environm, SKLLQG, Xian, Peoples R China
8.Vietnam Natl Univ Ho Chi Minh City, Fac Med, Otorhinolaryngol Dept, Ho Chi Minh City, Vietnam
9.Natl Taiwan Univ, Grad Inst Environm Engn, Taipei, Taiwan
10.Taipei Med Univ, Shuang Ho Hosp, Dept Internal Med, Div Pulm Med, New Taipei, Taiwan
11.Taipei Med Univ, Coll Med, Sch Resp Therapy, 250 Wuxing St, Taipei 110, Taiwan
12.Taipei Med Univ, Wan Fang Hosp, Cell Physiol & Mol Image Res Ctr, Taipei, Taiwan
Recommended Citation
GB/T 7714
Nguyen Thanh Tung,Ho, Kin-Fai,Niu, Xinyi,et al. Loss of E-cadherin due to road dust PM2.5 activates the EGFR in human pharyngeal epithelial cells[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2021:16.
APA Nguyen Thanh Tung.,Ho, Kin-Fai.,Niu, Xinyi.,Sun, Jian.,Shen, Zhenxing.,...&Chuang, Hsiao-Chi.(2021).Loss of E-cadherin due to road dust PM2.5 activates the EGFR in human pharyngeal epithelial cells.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,16.
MLA Nguyen Thanh Tung,et al."Loss of E-cadherin due to road dust PM2.5 activates the EGFR in human pharyngeal epithelial cells".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021):16.
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