Loss of E-cadherin due to road dust PM2.5 activates the EGFR in human pharyngeal epithelial cells

doi:10.1007/s11356-021-14469-2

IEECAS OpenIR > 粉尘与环境研究室

	Loss of E-cadherin due to road dust PM2.5 activates the EGFR in human pharyngeal epithelial cells
	Nguyen Thanh Tung 1,2; Ho, Kin-Fai 3; Niu, Xinyi 4; Sun, Jian 5; Shen, Zhenxing 5; Wu, Feng 6,7; Cao, Junji 6,7; Hoang Ba Dung 2; Tran Phan Chung Thuy 8; Hsiao, Ta-Chih 9; Liu, Wen-Te 10,11; Chuang, Hsiao-Chi 10,11,12
通讯作者	Liu, Wen-Te(lion5835@gmail.com) ; Chuang, Hsiao-Chi(chuanghc@tmu.edu.tw)
	2021-05-26
发表期刊	ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
ISSN	0944-1344
页码	16
摘要	Exposure to road dust particulate matter (PM) causes adverse health impacts on the human airway. However, the effects of road dust on the upper airway epithelium in humans remain unclear. We investigated the involvement of the epidermal growth factor receptor (EGFR) after PM with an aerodynamic diameter of < 2.5 mu m (PM2.5)-induced E-cadherin disruption of human pharyngeal epithelial cells. First, we collected road dust PM2.5 from 10 Chinese cities, including Wuhan, Nanjing, Shanghai, Guangzhou, Chengdu, Beijing, Lanzhou, Tianjin, Harbin, and Xi'an. Human pharyngeal FaDu cells were exposed to road dust PM2.5 at 50 mu g/mL for 24 h, cytotoxicity (cell viability and lactate dehydrogenase (LDH)) was assessed, and expressions of the proinflammatory interleukin (IL)-6 and high-mobility group box 1 (HMGB1) protein, receptor for advanced glycation end products (RAGE), occludin, E-cadherin, EGFR, and phosphorylated (p)-EGFR were determined. The E-cadherin gene was then knocked down to investigate EGFR activation in FaDu cells. Exposure to road dust PM2.5 resulted in a decrease in cell viability and increases in LDH and IL-6. Our data suggested that PM2.5 could decrease expressions of occludin and E-cadherin and increase expressions of EGFR and p-EGFR, which was confirmed by E-cadherin-knockdown. Our results showed a negative association between the alterations in E-cadherin and total elemental components in correlation analysis, especially S, Cl, K, Ti, Mn, Fe, Cu, Zn, and Pb. Exposure to metals in PM2.5 from road dust may lead to loss of the barrier function of the upper airway epithelium and activation of the EGFR. Our study showed the adverse effects of road dust PM2.5 on pharyngeal epithelial cells of the human upper airway.
关键词	Air pollution Allergy Chemical Metal Particulate matter Upper airway
DOI	10.1007/s11356-021-14469-2
关键词[WOS]	FINE PARTICULATE MATTER ; GROWTH-FACTOR RECEPTOR ; SOURCE PROFILES ; OXIDATIVE STRESS ; AIR-POLLUTION ; FUGITIVE DUST ; SOURCE APPORTIONMENT ; ALLERGIC RHINITIS ; MESENCHYMAL TRANSITION ; ELEMENTAL CARBON
收录类别	SCI ; SCI
语种	英语
资助项目	Ministry of Science and Technology of Taiwan[109-2314-B-038-093-MY3]
WOS研究方向	Environmental Sciences & Ecology
项目资助者	Ministry of Science and Technology of Taiwan
WOS类目	Environmental Sciences
WOS记录号	WOS:000654150700004
出版者	SPRINGER HEIDELBERG
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ieecas.cn/handle/361006/16706
专题	粉尘与环境研究室
通讯作者	Liu, Wen-Te; Chuang, Hsiao-Chi
作者单位	1.Taipei Med Univ, Coll Med, Int PhD Program Med, Taipei, Taiwan 2.Cho Ray Hosp, Otorhinolaryngol Dept, Ho Chi Minh City, Vietnam 3.Chinese Univ Hong Kong, JC Sch Publ Hlth & Primary Care, Hong Kong, Peoples R China 4.Xi An Jiao Tong Univ, Sch Human Settlements & Civil Engn, Xian, Peoples R China 5.Xi An Jiao Tong Univ, Dept Environm Sci & Engn, Xian, Peoples R China 6.Chinese Acad Sci, Inst Earth Environm, Key Lab Aerosol Chem & Phys, Xian, Peoples R China 7.Chinese Acad Sci, Inst Earth Environm, SKLLQG, Xian, Peoples R China 8.Vietnam Natl Univ Ho Chi Minh City, Fac Med, Otorhinolaryngol Dept, Ho Chi Minh City, Vietnam 9.Natl Taiwan Univ, Grad Inst Environm Engn, Taipei, Taiwan 10.Taipei Med Univ, Shuang Ho Hosp, Dept Internal Med, Div Pulm Med, New Taipei, Taiwan 11.Taipei Med Univ, Coll Med, Sch Resp Therapy, 250 Wuxing St, Taipei 110, Taiwan 12.Taipei Med Univ, Wan Fang Hosp, Cell Physiol & Mol Image Res Ctr, Taipei, Taiwan
推荐引用方式 GB/T 7714	Nguyen Thanh Tung,Ho, Kin-Fai,Niu, Xinyi,et al. Loss of E-cadherin due to road dust PM2.5 activates the EGFR in human pharyngeal epithelial cells[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2021:16.
APA	Nguyen Thanh Tung.,Ho, Kin-Fai.,Niu, Xinyi.,Sun, Jian.,Shen, Zhenxing.,...&Chuang, Hsiao-Chi.(2021).Loss of E-cadherin due to road dust PM2.5 activates the EGFR in human pharyngeal epithelial cells.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,16.
MLA	Nguyen Thanh Tung,et al."Loss of E-cadherin due to road dust PM2.5 activates the EGFR in human pharyngeal epithelial cells".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021):16.