Antibiotic resistance gene transfer during anaerobic digestion with added copper: Important roles of mobile genetic elements

doi:10.1016/j.scitotenv.2020.140759

IEECAS OpenIR > 黄土与第四纪地质国家重点实验室（2010~）

	Antibiotic resistance gene transfer during anaerobic digestion with added copper: Important roles of mobile genetic elements
	Zhang, Ranran 1,2; Gu, Jie 2; Wang, Xiaojuan 2; Li, Yang3
通讯作者	Gu, Jie(gujie205@sina.com)
	2020-11-15
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
卷号	743 页码:8
摘要	The abuse of heavy metals as feed additives in livestock is widespread and it might aggravate the spread of antibiotic resistance genes (ARGs) Lt the environment. However, the mechanisms that allow heavy metals to increase the transmission of ARGs in the environment remain unclear. Cu is the heavy metal present at the highest concentration in livestock manure, and thus Cu was selected to investigate the responses of ARGs to heavy metals. The effects of the microbial communities, mobile genetic elements (MGEs), and heavy metal resistance genes (HMRGs) on ARGs were determined in the presence of 75 and 227 mg L-1 Cu in a swine manure anaerobic digestion (AD) system. In the AD products, the presence of residual Cu (75 and 227 mg L-1) increased the total ARGs, HMRGs, and some MGEs, and the higher Cu selected more ARGs than the lower Cu treatment. The results demonstrated that Cu could promote the co-selection of HMRGs, ARGs, and MGEs. The different levels of Cu did not change the bacterial community composition, but they influenced the abundances of bacteria during AD. Network analysis showed that the presence of Cu increased the co-occurrence of specific bacteria containing ARGs, HMRGs, and MGEs. Furthermore, the co-occurrence of MGEs and ARG5 increased greatly compared with that of HMRGs and ARGs. Therefore, compared HMRGs, the increased MGEs had the main effect on increasing of ARGs. (C) 2020 Published by Elsevier B.V.
关键词	Bacterial community Co-occurrence Copper resistance gene Mobile genetic element Network analysis
DOI	10.1016/j.scitotenv.2020.140759
关键词[WOS]	SWINE-MANURE ; ANTIMICROBIAL RESISTANCE ; BACTERIAL COMMUNITY ; AGRICULTURAL SOILS ; ANIMAL PRODUCTION ; HEAVY-METALS ; WASTE-WATER ; ABUNDANCE ; MECHANISMS ; BIOGAS
收录类别	SCI ; SCI
语种	英语
资助项目	National Natural Science Foundation of China[41671474] ; National Natural Science Foundation of China[41601531] ; Science and Technology Plan Key Project of Shaanxi province[2017ZDCXL-SF-03-03]
WOS研究方向	Environmental Sciences & Ecology
项目资助者	National Natural Science Foundation of China ; Science and Technology Plan Key Project of Shaanxi province
WOS类目	Environmental Sciences
WOS记录号	WOS:000573545300008
出版者	ELSEVIER
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ieecas.cn/handle/361006/15358
专题	黄土与第四纪地质国家重点实验室（2010~）
通讯作者	Gu, Jie
作者单位	1.Northwestern Polytech Univ, Sch Ecol & Environm, Xian 710129, Shaanxi, Peoples R China 2.Northwest A&F Univ, Coll Nat Resources & Environm, Yangling 712100, Shaanxi, Peoples R China 3.Chinese Acad Sci, Inst Earth Environm, SKLLQG, KLACP, Xian 710061, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ranran,Gu, Jie,Wang, Xiaojuan,et al. Antibiotic resistance gene transfer during anaerobic digestion with added copper: Important roles of mobile genetic elements[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,743:8.
APA	Zhang, Ranran,Gu, Jie,Wang, Xiaojuan,&Li, Yang.(2020).Antibiotic resistance gene transfer during anaerobic digestion with added copper: Important roles of mobile genetic elements.SCIENCE OF THE TOTAL ENVIRONMENT,743,8.
MLA	Zhang, Ranran,et al."Antibiotic resistance gene transfer during anaerobic digestion with added copper: Important roles of mobile genetic elements".SCIENCE OF THE TOTAL ENVIRONMENT 743(2020):8.