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The effect of processing medium on the H-2/H-1 of carbon-bound hydrogen in alpha-cellulose extracted from higher plants
Zhu, Zhenyu1,2,5; Yin, Xijie3; Lu, Fengyan4; Wang, Bo1,2; Ma, Ran1,2; Zhao, Yu1,2; Wang, Ying1,2; Ma, Yi1,2; Su, Jing3; Yan, Qiulin1,2; Hocart, Charles H.1,2,6; Zhou, Youping1,2,5
通讯作者Zhou, Youping(youping.zhou@sust.edu.cn)
2020-05-30
发表期刊RAPID COMMUNICATIONS IN MASS SPECTROMETRY
ISSN0951-4198
卷号34期号:10页码:12
摘要Rationale Although the H-2/H-1 ratio of the carbon-bound hydrogens (C-Hs) in alpha-cellulose extracted from higher plants has long been used successfully for climate, environmental and metabolic studies, the assumption that bleaching with acidified NaClO2 to remove lignin before pure alpha-cellulose can be obtained does not alter the H-2/H-1 ratio of alpha-cellulose C-Hs has nonetheless not been tested. Methods For reliable application of the H-2/H-1 ratio of alpha-cellulose C-H, we processed plant materials representing different phytochemistries and photosynthetic carbon assimilation modes in isotopically contrasting bleaching media (with an isotopic difference of 273 mUr). All the isotope ratios were measured by elemental analyzer/isotope ratio mass spectrometry (EA/IRMS). Results Our results show that H from the bleaching medium does appear in the final pure alpha-cellulose product, although the isotopic alteration to the C-H in alpha-cellulose due to the incorporation of processing H from the medium is small if isotopically "natural" water is used to prepare the processing medium. However, under prolonged bleaching such an isotope effect can be significant, implying that standardizing the bleaching process is necessary for reliable H-2/H-1 measurement. Conclusions The currently adopted method for removing lignin for alpha-cellulose extraction from higher plant materials with acidified NaClO2 bleaching is considered acceptable in terms of preserving the isotopic fidelity if isotopically "natural" water is used to prepare the bleaching solution.
DOI10.1002/rcm.8641
关键词[WOS]CONTAINING CHEMICAL LINKAGES ; DELTA-D VALUES ; D/H RATIOS ; NONEXCHANGEABLE HYDROGEN ; TREE-RINGS ; OXYGEN ; FRACTIONATION ; LIGNIN ; EXCHANGE ; WATER
收录类别SCI ; SCI
语种英语
资助项目Chinese NSF[41773032] ; Chinese NSF[41973072] ; Shaanxi Natural Science Basic Research Plan[2019JQ-232] ; Shaanxi Natural Science Basic Research Plan[037]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
项目资助者Chinese NSF ; Shaanxi Natural Science Basic Research Plan
WOS类目Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy
WOS记录号WOS:000529873700017
出版者WILEY
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ieecas.cn/handle/361006/14755
专题现代环境研究室
通讯作者Zhou, Youping
作者单位1.Shaanxi Univ Sci & Technol, Sch Chem & Chem Engn, Isotop Chem Biol, Weiyang Univ Pk, Xian 710021, Peoples R China
2.Shaanxi Univ Sci & Technol, Sch Chem & Chem Engn, Shaanxi Key Lab Chem Addit Ind, Weiyang Univ Pk, Xian 710021, Peoples R China
3.MNR Third Inst Oceanol, Lab Marine & Coastal Geol, Xiamen 361005, Peoples R China
4.Chinese Acad Sci, Inst Earth Environm, Xian 710061, Peoples R China
5.Southern Marine Sci & Engn Guangdong Lab, Zhuhai 519000, Peoples R China
6.Australian Natl Univ, Res Sch Biol, GPO Box 4, Canberra, ACT 2601, Australia
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Zhu, Zhenyu,Yin, Xijie,Lu, Fengyan,et al. The effect of processing medium on the H-2/H-1 of carbon-bound hydrogen in alpha-cellulose extracted from higher plants[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2020,34(10):12.
APA Zhu, Zhenyu.,Yin, Xijie.,Lu, Fengyan.,Wang, Bo.,Ma, Ran.,...&Zhou, Youping.(2020).The effect of processing medium on the H-2/H-1 of carbon-bound hydrogen in alpha-cellulose extracted from higher plants.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,34(10),12.
MLA Zhu, Zhenyu,et al."The effect of processing medium on the H-2/H-1 of carbon-bound hydrogen in alpha-cellulose extracted from higher plants".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 34.10(2020):12.
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