Evaluation of epifluorescence methods for quantifying bioaerosols in fine and coarse particulate air pollution

doi:10.1016/j.atmosenv.2019.05.051

IEECAS OpenIR > 粉尘与环境研究室

	Evaluation of epifluorescence methods for quantifying bioaerosols in fine and coarse particulate air pollution
	Chen, L-W Antony 1,2; Zhang, Mi 2; Liu, Ting 3; Fortier, Karey 1; Chow, Judith C.2,4; Alonzo, Fernanda 1; Kolberg, Rachel 1,5; Cao, Junji 4; Lin, Ge 1; Patel, Tanviben Y.1; Cruz, Patricia 1; Buttner, Mark P.1; Watson, John G.2,4
通讯作者	Chen, L-W Antony(antony.chen@unlv.edu)
	2019-09-15
发表期刊	ATMOSPHERIC ENVIRONMENT
ISSN	1352-2310
卷号	213 页码:620-628
摘要	Despite being recognized as an important part of particulate matter (PM) air pollution and health risk, bioaerosols have not been quantified as extensively as other PM components for establishing PM standards and management strategies. The challenge lies partly in the lack of practical measurement methods. This study evaluated a filter-based, direct-staining fluorescence microscopy (DS-FM) method that may be adapted to routine air quality monitoring for bioaerosol concentration and size distribution. Through testing with bioaerosol standards made of bacterial cells and fungal spores, the method is shown to have precision, accuracy, detection limit, and dynamic range suitable for most ambient environments. DS-FM was demonstrated with PM samples from an arid urban location in Las Vegas, Nevada during the spring allergy season. Detectable bioaerosols ranged from 0.37 to 16 mu m in geometric diameter and averaged 0.27 +/- 0.23 cm(-3) in number concentration with about 2/3 and 1/3 in the fine (<= 2.5 mu m) and coarse (> 2.5 mu m) mode, respectively. The bioaerosol mass, estimated from the size distribution and an assumed density, was mainly in the coarse mode and accounted for 17 +/- 11% of PM10, 20 +/- 13% of PM10-2.5, and 4 +/- 3% of PM2.5 mass. Rain and high wind speeds appeared to elevate bioaerosol levels. Other advantages of DS-FM include low sample consumption and short turnaround times; a large amount of data can be generated by incorporating the measurement into current long-term air quality networks. Suggestions for using the data to inform bioaerosol origins, contributions, and public health impacts are discussed.
关键词	Primary biological aerosol particles Automated particle counting Aerosol size distribution DNA stain PM10 PMcoarse
DOI	10.1016/j.atmosenv.2019.05.051
关键词[WOS]	BIOLOGICAL AEROSOL-PARTICLES ; TOTAL AIRBORNE MICROBES ; SIZE DISTRIBUTIONS ; ORGANIC-CARBON ; CLOUD-WATER ; UV-APS ; HEALTH ; MICROSCOPY ; RAIN ; BACTERIA
收录类别	SCI ; SCI
语种	英语
资助项目	School of Public Health, University of Nevada, Las Vegas ; Environmental Analysis Facility, Desert Research Institute ; Hubei Province of China[D20184502] ; Clark County School District
WOS研究方向	Environmental Sciences & Ecology ; Meteorology & Atmospheric Sciences
项目资助者	School of Public Health, University of Nevada, Las Vegas ; Environmental Analysis Facility, Desert Research Institute ; Hubei Province of China ; Clark County School District
WOS类目	Environmental Sciences ; Meteorology & Atmospheric Sciences
WOS记录号	WOS:000484870900059
出版者	PERGAMON-ELSEVIER SCIENCE LTD
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ieecas.cn/handle/361006/13424
专题	粉尘与环境研究室
通讯作者	Chen, L-W Antony
作者单位	1.Univ Nevada, Sch Publ Hlth, Dept Environm & Occupat Hlth, Las Vegas, NV 89154 USA 2.Desert Res Inst, Div Atmospher Sci, Reno, NV 89512 USA 3.Hubei Polytech Univ, Sch Environm Sci & Engn, Hubei Key Lab Mine Environm Pollut Control & Reme, Huangshi 435003, Hubei, Peoples R China 4.Chinese Acad Sci, Inst Earth Environm, KLACP, Xian 710061, Shaanxi, Peoples R China 5.Clark Cty Dept Air Qual, Las Vegas, NV 89118 USA
推荐引用方式 GB/T 7714	Chen, L-W Antony,Zhang, Mi,Liu, Ting,et al. Evaluation of epifluorescence methods for quantifying bioaerosols in fine and coarse particulate air pollution[J]. ATMOSPHERIC ENVIRONMENT,2019,213:620-628.
APA	Chen, L-W Antony.,Zhang, Mi.,Liu, Ting.,Fortier, Karey.,Chow, Judith C..,...&Watson, John G..(2019).Evaluation of epifluorescence methods for quantifying bioaerosols in fine and coarse particulate air pollution.ATMOSPHERIC ENVIRONMENT,213,620-628.
MLA	Chen, L-W Antony,et al."Evaluation of epifluorescence methods for quantifying bioaerosols in fine and coarse particulate air pollution".ATMOSPHERIC ENVIRONMENT 213(2019):620-628.