Ecoenzymatic stoichiometry reveals microbial phosphorus limitation decreases the nitrogen cycling potential of soils in semi-arid agricultural ecosystems

doi:10.1016/j.still.2019.104463

IEECAS OpenIR > 生态环境研究室

	Ecoenzymatic stoichiometry reveals microbial phosphorus limitation decreases the nitrogen cycling potential of soils in semi-arid agricultural ecosystems
	Cui, Yongxing 1,3; Zhang, Yanle 1,3; Duan, Chengjiao 1,3; Wang, Xia 1,3; Zhang, Xingchang 1; Ju, Wenliang 1,3; Chen, Hansong 3,5; Yue, Shanchao 1; Wang, Yunqiang 2,4; Li, Shiqing 1; Fang, Linchuan 1,2
通讯作者	Fang, Linchuan(flinc629@hotmail.com)
	2020-03-01
发表期刊	SOIL & TILLAGE RESEARCH
ISSN	0167-1987
卷号	197 页码:10
摘要	Variations in soil microbial metabolism currently represent one of the greatest areas of uncertainty with regard to soil nutrient cycles and the control of terrestrial carbon (C) and nitrogen (N) loss and are poorly understood in agricultural ecosystems with intensive farming practices. In this study, extracellular enzymatic stoichiometry models and quantitative PCR techniques were used to examine microbial metabolic limitation and its relationship with N-cycling gene expression in semi-arid agricultural ecosystems considering four N fertilization levels (N 0, N 100, N 250, and N 400 kg N ha(-1)) and two agronomic strategies (film mulching and no mulching). Film mulching increased microbial C limitation (reflecting microbial C metabolism size; 0.189 of the total effects), while very small effects on microbial phosphorus (P) limitation were observed (-0.007 of the total effects). N fertilization increased the microbial demand for P (microbial P limitation; 0.504 of the total effects). Increased microbial C metabolism was mainly attributed to increased soil moisture content after film mulching, which enhanced microbial decomposition of organic C (high C-acquiring enzyme activities). Changes in nutrient stoichiometry and the increase in N availability due to N fertilization were largely responsible for increased microbial P limitation. Furthermore, microbial P limitation negatively affected the abundance of AOA amoA, AOB amoA (involved in nitrification), nirK, nirS, nosZ (involved in denitrification) genes, strongly inhibiting nitrification and denitrification potential (-0.743 and -0.761 of the total effects, respectively). The present results suggest that agricultural ecosystems with film mulching are conducive to organic residue decomposition, while appropriate P limitation under N fertilization could reduce the loss of N due to nitrification and denitrification in soil. This study highlights the importance of elemental stoichiometry-driven microbial metabolic variation in understanding soil nutrient cycles and optimizing agricultural practices.
关键词	Extracellular enzyme activity Microbial metabolic limitation Functional genes
DOI	10.1016/j.still.2019.104463
关键词[WOS]	AMMONIA-OXIDIZING BACTERIA ; ORGANIC-CARBON ; LOESS PLATEAU ; FERTILIZATION ; COMMUNITIES ; MOISTURE ; RESPIRATION ; TEMPERATURE ; ABUNDANCE ; FLEXIBILITY
收录类别	SCI ; SCI
语种	英语
资助项目	National Natural Science Foundation of China[41977031] ; CAS Light of West China Program[XAB2016A03] ; Program of State Key Laboratory of Loess and Quaternary Geology CAS[SKLLQGZR1803] ; State Key Research & Development Plan Project[2017YFC0504504] ; Education Department Foundation of Zhejiang Province[Y201738652]
WOS研究方向	Agriculture
项目资助者	National Natural Science Foundation of China ; CAS Light of West China Program ; Program of State Key Laboratory of Loess and Quaternary Geology CAS ; State Key Research & Development Plan Project ; Education Department Foundation of Zhejiang Province
WOS类目	Soil Science
WOS记录号	WOS:000514003800009
出版者	ELSEVIER
引用统计	被引频次：87[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ieecas.cn/handle/361006/12728
专题	生态环境研究室第四纪科学与全球变化卓越创新中心
通讯作者	Fang, Linchuan
作者单位	1.CAS & MWR, State Key Lab Soil Eros & Dryland Farming Loess P, Inst Soil & Water Conservat, Yangling 712100, Shaanxi, Peoples R China 2.CAS Ctr Excellence Quaternary Sci & Global Change, Xian 710061, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, State Key Lab Loess & Quaternary Geol, Inst Earth Environm, Xian 710061, Peoples R China 5.Zhejiang Normal Univ, Xingzhi Coll, Jinhua 321004, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Cui, Yongxing,Zhang, Yanle,Duan, Chengjiao,et al. Ecoenzymatic stoichiometry reveals microbial phosphorus limitation decreases the nitrogen cycling potential of soils in semi-arid agricultural ecosystems[J]. SOIL & TILLAGE RESEARCH,2020,197:10.
APA	Cui, Yongxing.,Zhang, Yanle.,Duan, Chengjiao.,Wang, Xia.,Zhang, Xingchang.,...&Fang, Linchuan.(2020).Ecoenzymatic stoichiometry reveals microbial phosphorus limitation decreases the nitrogen cycling potential of soils in semi-arid agricultural ecosystems.SOIL & TILLAGE RESEARCH,197,10.
MLA	Cui, Yongxing,et al."Ecoenzymatic stoichiometry reveals microbial phosphorus limitation decreases the nitrogen cycling potential of soils in semi-arid agricultural ecosystems".SOIL & TILLAGE RESEARCH 197(2020):10.